Fe(III) stimulates 3-methylindole and 4-methylphenol production in swine lagoon enrichments and Clostridium scatologenes ATCC 25775.

Draft Genome Sequence of Clostridium scatologenes ATCC 25775, a Chemolithoautotrophic Acetogenic Bacterium Producing 3-Methylindole and 4-Methylphenol

Clostridium scatologenes ATCC 25775 is a strictly anaerobic and chemolithoautotrophic acetogenic bacterium that converts syngas into multi-carbon compounds such as acetate, indole, 3-methylindole, and 4-methylphenol. Here we report the draft genome sequence of C. scatologenes ATCC 25775 (7.3 Mbp) to elucidate its metabolic pathway for syngas fermentation.

3-Methylindole production is regulated in Clostridium scatologenes ATCC 25775.

AIMS 3-Methylindole (3-MI) is a degradation product of L-tryptophan and is both an animal waste malodorant and threat to ruminant health. Culture conditions influencing 3-MI production in Clostridium scatologenes ATCC 25775 were investigated. METHODS AND RESULTS Extracellular 3-MI levels in cells cultured in brain heart infusion (BHI) medium (pH 7.0) at 33 degrees C and 37 degrees C for 72 h ...

Development of a Genetic Modification System in <i>Clostridium scatologenes</i> ATCC 25775 for Generation of Mutants

Catabolic pathway for the production of skatole and indoleacetic acid by the acetogen Clostridium drakei, Clostridium scatologenes, and swine manure.

Skatole (3-methylindole) is a malodorous chemical in stored swine manure and is implicated as a component of foul-tasting pork. Definitive evidence for the skatole pathway is lacking. Deuterium-labeled substrates were employed to resolve this pathway in the acetogenic bacterium Clostridium drakei and Clostridium scatologenes and to determine if a similar pathway is used by microorganisms presen...

Clostridium nitrophenolicum sp. nov., a novel anaerobic p-nitrophenol-degrading bacterium, isolated from a subsurface soil sample.

An obligate anaerobic, mesophilic, motile and endospore-forming bacterium, designated 1D(T), was isolated from a subsurface soil sample. The young culture of strain 1D(T) was Gram-positive and formed oval spores that were central in position. Based on the biochemical, chemotaxonomic and physiological data, strain 1D(T) appears to be a member of the genus Clostridium. Strain 1D(T) was found to b...